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KMID : 0385219960060010052
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Korean Journal of Gerontology
1996 Volume.6 No. 1 p.52 ~ p.57
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Role of osterotrophic hormones on osteoclast generation in coculture of bone marrow cells and osteoblastic cells
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Kim Hyung-Ryong
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Abstract
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Several evidences have recently suggested that osteoblasts are involved not only in bone formation but also in bone resorption. The idea that cells of osterotrophic lineage are in bone resorption derives from the fact that receptors for bone-resorbing hormones, such as parathyroid hormone(PTH) and 1α,25-dihydroxyvitamin D3(1,25-[OH]2D3), are present in osteoblast but not in osteoclast. Osteoclast are formed by fusion of monouclear precusors derived from hematopoitic progenitor cell. Since the osteoclast in hemopeitic in origin, it is reasonable that bone marrow culture systems can be used to study osteoclast formation. Therefore, this experiment developed a coculture system of mouse bone marrow cells with osteoblastic clone MC3T3E1 cells to examine the possible involvement of osteoblast in osterclast generation in the presence or absence of PTH and prostaglandin E2(PGE2). After cultures, staining for tartrate-resistant acid phosphatase(TRAP)-marker enzyme of osteoclast-was performed according to the modified method of Burstone. The TRAP-positive multinucleated cells which have 3 or more nuclei were counted. The observed results were as follow, In control group, TRAP-positive mononucler cells were present, but no TRAP-positive multinucleated cells appeared. More TRAP-positive multinucleated cells were formed in coculture system than in control group. PTH and PGE2 stimulate the formation of osteoclast-like cells from mouse bone marrow cells in coculture. PTH and PGE2 stimulate the formation osteoclast-like cells from mouse bone marrow cells in coculture of osteoblast-like cells.
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KEYWORD
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PTH, PGE2, Bone marrow cell, MC3T3E1, Osteoclast generation
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